Expression of unknown genes
Methods such as EST analysis, SAGE, and DNA arrays are all used for studying transcriptomes, but these all yield only limited information about the genes in question. Hybridization is also a possibility, but sensitivity is limited.
HiCEP technology fills this gap by allowing comparison and detection of genes with differing levels of transcription product expressed between multiple samples regardless of whether or not sequence information is known. When necessary, we can decode the sequence for the target gene.
Excellent genetic analysis capabilities
HiCEP technology does not depend on knowledge accumulated about any given gene. It allow amplification and fragment peak detection without altering the obtained gene population. Each peak corresponds to one gene, so changes in expression level may be identified.
It is thought that over 20,000 genes are expressed in each cell, although the quantity depends on the cell type.
HiCEP technology can detect over 15,000 of these genes, or 80-85%, enabling the construction of broad expression networks.
Rare genes also detectable
Fragment analysis lies at the foundation of HiCEP technology. This both permits high detection sensitivity (differentials as low as 1.5X) and makes the technology highly effective at analysis of rarely expressed genes because it uses peak height to detect expression level changes.
Expression profiles can be compared among experiments during systematic studies of single cells or organisms (stimulus response, longitudinal experiments, etc.). Since the technology does not require control samples, researchers may reduce the number of experiments without effecting data quality, thereby controlling cost.